Introduction to Flow Cytometry and its Applications-3rd Edition
- Types
- Online
- Start date
- Tuesday, April 06, 2021
- End date
- Sunday, April 18, 2021
- Teachers
Anaïs Panosa, Flow Cytometry Facility technical lead and Alba Garcia, technician
Target Audience:
To all IRBLleida Community including researchers, students, phisycians, resident phisycians and UdL students and community.
Objectives:
- Introduce the fundamental basics of Flow Cytometry
- Explain different applications in Flow Cytometry
- Introduce the IRBLleida Flow Cytometry Facility and its services.
Methodology:
Online presentation with Q&A section and a practical session in the Cytomics Lab (Lab Aux. 4.1 4a planta, bio2).
Evaluation:
In order to obtain the course certification you need to attend to both online and practical session. Concept assimilation will be evaluated by Kahoot tests administered during the online presentation.
Date:
- Online Presentation: 27th April 2021 from 10h to 13h.
- Practical Session (Lab. Aux. 4.1 4a planta Bio2): To be determined depending on the number of registered attendants (duration: 2 hours).
Programme:
1- Flow cytometry basics
Introduction to flow cytometer parts: fluidics, optics and electronics. Data analyisis.
2- Working with fluorochromes and antibodies
Staining methodologies, Fluorochromes and Antibodies
3- Flow Cytometry experiment set up
How to properly set up a new experiment.
4- Applications of Flow Cytometry
Clinical applications, Immunophenotiping, Apoptosis, Cell cycle, Functional analysis (oxidative stress, calcium flux, cell proliferation, FRET, etc...), Bead arrays, Exosome analysis
5- Cell Sorting and applications
Sorting technologies. Important concepts in cell sorting. Applications.
6- Imaging Flow Cytometry and applications
Imaging Flow Cytometry Technology. Applications
7- New applications in FC: Mass Cytometry, Spectral Flow cytometry
Mass Cytometry Technology and applications in biomedicine.
8- IRBLleida Flow Cytometry Facility
9- Practical session in the Flow Cytometry Lab
· How to design a multicolour experiment: Cytometer Set Up and compensation
· Immunophenotyping experiment
· Cell cycle experiment
· Apoptosis experiment
Registration: https://www.irblleida.org/ca/inscripcio-curs-citometria/
Once registered, you should answer this short questionnaire (link below) in order to know which applications you are most interested in so we can adjust to your preferences in the online presentation. Registration Limit: 30 attendants in a first come first served basis.
