Microscopy and Cytometry
The Microscopy and Cytometry Service is divided into two locations, one for the Flow Cytometry Unit and the other for the Confocal Microscopy Unit. Both units are located in the basement of the Biomedicine building, in laboratories B-1.5 and B-1.1 respectively.
The Flow Cytometry Unit currently has a digital analyzer cytometer FACS Canto-II (from Becton Dickinson) with three lasers 4-2-2 configuration and to analyze up to eight different colours. The cytometer is equipped with a computer to control the operation and comes installed with FacsDiva v. 6.1.1 analysis software. Also available is a magnetic cell separator device, Automac Pro (Miltenyi Biotec) in a cabin class II biosafety.
In addition, the service also offers additional software for different applications such as ModFit LT for the analysis of cell cycle FCAP Array experiments CBA (BD) and two analysis software file licenses for FlowLogic cytometry (Inivai). Other facilities available in the Cytometry Service are: a 4oC refrigerator, a vortex, a set of micropipettes, a printer, and computer independent analysis data.
Now we are offering: cell cycle studies, oxidative stress (various fluorescent probes) studies for immunophenotyping, apoptosis (annexin V, caspase 6, phosphorylated histone H3, etc ...), analysis concentration of cytokines by cytometric bead array (CBA BD), among others.