The objectives of this platform are the following:
1. Detect, identify and qualify all the lipid species of a system (cells, tissues, organs, food, etc.)
2. Define lipid profiles in physiological and pathological conditions
3. Identify new lipid biomarkers
To achieve these objectives, the lipidomic platform offers technical support and advice to research groups that request it on possible experimental applications, as well as the processing of samples for each of the applications. We prepare and optimize the protocols for the different analyzes required by the users and we also take care of the processing and analysis of data at the request of the user.
The lipidomic platform consists of two HPLC units (Cap Pump 1200 Serves G1376A, Nano Pump 1200 Series G2226A, Agilent Technologies), two UPLC units (Agilent 1290 Infinity Quaternary Pump), two QTOF mass detection systems (QTOF LC/MS G6520A and 6545, Agilent Technologies), a mass detection system TQD (Triple Quad 6420 LC/MS Agilent Technologies), an ionization system in nanoelectrosprai using Chip Cube technology (HPLC Chip Cube G4240A, Agilent Technologies) and the necessary software for analysis bioinformatic of the metabolic data generated in said platform (MassHunter Data Acquisition, MassHunter Qualitative Analysis, MassHunter Quantitative Analysis, MassHunter Mass Profiler, Agilent Technologies). The platform is located partially in the Institute of Biomedical Research of Lleida, Building Biomedicina 2, 4th floor, and partially in the H3 of the Agri-Food Science and Technology Park of Lleida (PCiTAL), in the space called “Platform of Scientific and Technical Services".